Gazing at the Solar System: Capturing the Evolution of Dunes, Faults, Volcanoes, and Ice from Space

Gazing imaging holds promise for improved understanding of surface characteristics and processes of Earth and solar system bodies. Evolution of earthquake fault zones, migration of sand dunes, and retreat of ice masses can be understood by observing changing features over time. To gaze or stare means to look steadily, intently, and with fixed attention, offering the ability to probe the characteristics of a target deeply, allowing retrieval of 3D structure and changes on fine and coarse scales. Observing surface reflectance and 3D structure from multiple perspectives allows for a more complete view of a surface than conventional remote imaging. A gaze from low Earth orbit (LEO) could last several minutes allowing for video capture of dynamic processes. Repeat passes enable monitoring time scales of days to years. Numerous vantage points are available during a gaze (Figure 1). Features in the scene are projected into each image frame enabling the recovery of dense 3D structure. The recovery is robust to errors in the spacecraft position and attitude knowledge, because features are from different perspectives. The combination of a varying look angle and the solar illumination allows recovering texture and reflectance properties and permits the separation of atmospheric effects. Applications are numerous and diverse, including, for example, glacier and ice sheet flux, sand dune migration, geohazards from earthquakes, volcanoes, landslides, rivers and floods, animal migrations, ecosystem changes, geysers on Enceladus, or ice structure on Europa. The Keck Institute for Space Studies (KISS) hosted a workshop in June of 2014 to explore opportunities and challenges of gazing imaging. The goals of the workshop were to develop and discuss the broad scientific questions that can be addressed using spaceborne gazing, specific types of targets and applications, the resolution and spectral bands needed to achieve the science objectives, and possible instrument configurations for future missions. The workshop participants found that gazing imaging offers the ability to measure morphology, composition, and reflectance simultaneously and to measure their variability over time. Gazing imaging can be applied to better understand the consequences of climate change and natural hazards processes, through the study of continuous and episodic processes in both domains

Gazing at the Solar System: Capturing the Evolution of Dunes, Faults, Volcanoes, and Ice from Space

Gazing imaging holds promise for improved understanding of surface characteristics and processes of Earth and solar system bodies. Evolution of earthquake fault zones, migration of sand dunes, and retreat of ice masses can be understood by observing changing features over time. To gaze or stare means to look steadily, intently, and with fixed attention, offering the ability to probe the characteristics of a target deeply, allowing retrieval of 3D structure and changes on fine and coarse scales. Observing surface reflectance and 3D structure from multiple perspectives allows for a more complete view of a surface than conventional remote imaging. A gaze from low Earth orbit (LEO) could last several minutes allowing for video capture of dynamic processes. Repeat passes enable monitoring time scales of days to years. Numerous vantage points are available during a gaze (Figure 1). Features in the scene are projected into each image frame enabling the recovery of dense 3D structure. The recovery is robust to errors in the spacecraft position and attitude knowledge, because features are from different perspectives. The combination of a varying look angle and the solar illumination allows recovering texture and reflectance properties and permits the separation of atmospheric effects. Applications are numerous and diverse, including, for example, glacier and ice sheet flux, sand dune migration, geohazards from earthquakes, volcanoes, landslides, rivers and floods, animal migrations, ecosystem changes, geysers on Enceladus, or ice structure on Europa. The Keck Institute for Space Studies (KISS) hosted a workshop in June of 2014 to explore opportunities and challenges of gazing imaging. The goals of the workshop were to develop and discuss the broad scientific questions that can be addressed using spaceborne gazing, specific types of targets and applications, the resolution and spectral bands needed to achieve the science objectives, and possible instrument configurations for future missions. The workshop participants found that gazing imaging offers the ability to measure morphology, composition, and reflectance simultaneously and to measure their variability over time. Gazing imaging can be applied to better understand the consequences of climate change and natural hazards processes, through the study of continuous and episodic processes in both domains

DNA repair during nonreductional meiosis in the asexual rotifer Adineta vaga

Rotifers of the class Bdelloidea are microscopic animals notorious for their long-term persistence in the apparent absence of sexual reproduction and meiotic recombination. This evolutionary paradox is often counterbalanced by invoking their ability to repair environmentally induced genome breakage. By studying the dynamics of DNA damage response in the bdelloid species , we found that it occurs rapidly in the soma, producing a partially reassembled genome. By contrast, germline DNA repair is delayed to a specific time window of oogenesis during which homologous chromosomes adopt a meiotic-like juxtaposed configuration, resulting in accurate reconstitution of the genome in the offspring. Our finding that a noncanonical meiosis is the mechanism of germline DNA repair in bdelloid rotifers gives previously unidentified insights on their enigmatic long-term evolution

Control of directionality in the DNA strand-exchange reaction catalysed by the tyrosine recombinase TnpI

In DNA site-specific recombination catalysed by tyrosine recombinases, two pairs of DNA strands are sequentially exchanged between separate duplexes and the mechanisms that confer directionality to this theoretically reversible reaction remain unclear. The tyrosine recombinase TnpI acts at the internal resolution site (IRS) of the transposon Tn4430 to resolve intermolecular transposition products. Recombination is catalysed at the IRS core sites (IR1–IR2) and is regulated by adjacent TnpI-binding motifs (DR1 and DR2). These are dispensable accessory sequences that confer resolution selectivity to the reaction by stimulating synapsis between directly repeated IRSs. Here, we show that formation of the DR1–DR2-containing synapse imposes a specific order of activation of the TnpI catalytic subunits in the complex so that the IR1-bound subunits catalyse the first strand exchange and the IR2-bound subunits the second strand exchange. This ordered pathway was demonstrated for a complete recombination reaction using a TnpI catalytic mutant (TnpI-H234L) partially defective in DNA rejoining. The presence of the DR1- and DR2-bound TnpI subunits was also found to stabilize transient recombination intermediates, further displacing the reaction equilibrium towards product formation. Implication of TnpI/IRS accessory elements in the initial architecture of the synapse and subsequent conformational changes taking place during strand exchange is discussed

Ice needles weave patterns of stones in freezing landscapes

Self-organization is increasingly recognized as fundamental to pattern formation in geomorphology. Relative to other fields, however, underlying mechanisms have received little attention from theoreticians. Here, we introduce phase separation theory to study the formation of sorted patterned ground in cold regions; textquotedblleftsortedtextquotedblright refers to the segregation of soil and stones due to feedbacks between stone concentration and recurring ice growth. Using detailed measurements of the concentration of stones in soil and their displacements, we demonstrate that phase separation accounts for the observed sorting and patterns. Our study highlights phase separation theory as a source of important insight into studying ground patterns in cold regions and their potential value in signaling important changes in ground conditions with the warming climate.Patterned ground, defined by the segregation of stones in soil according to size, is one of the most strikingly self-organized characteristics of polar and high-alpine landscapes. The presence of such patterns on Mars has been proposed as evidence for the past presence of surface liquid water. Despite their ubiquity, the dearth of quantitative field data on the patterns and their slow dynamics have hindered fundamental understanding of the pattern formation mechanisms. Here, we use laboratory experiments to show that stone transport is strongly dependent on local stone concentration and the height of ice needles, leading effectively to pattern formation driven by needle ice activity. Through numerical simulations, theory, and experiments, we show that the nonlinear amplification of long wavelength instabilities leads to self-similar dynamics that resemble phase separation patterns in binary alloys, characterized by scaling laws and spatial structure formation. Our results illustrate insights to be gained into patterns in landscapes by viewing the pattern formation through the lens of phase separation. Moreover, they may help interpret spatial structures that arise on diverse planetary landscapes, including ground patterns recently examined using the rover Curiosity on Mars.The experimental data analyzed during this study are available in the manuscript and SI Appendix files. All custom-made simulation codes are available online at GitHub: https://github.com/liuqx315/Phase-separation-patterned-ground. All other study data are included in the article and/or supporting information

Enantioselective regulation of lactate racemization by LarR in Lactobacillus plantarum.

Lactobacillus plantarum is a lactic acid bacterium that produces a racemic mixture of l- and d-lactate from sugar fermentation. The interconversion of lactate isomers is performed by a lactate racemase (Lar) that is transcriptionally controlled by the l-/d-lactate ratio and maximally induced in the presence of l-lactate. We previously reported that the Lar activity depends on the expression of two divergently oriented operons: (i) the larABCDE operon encodes the nickel-dependent lactate racemase (LarA), its maturases (LarBCE), and a lactic acid channel (LarD), and (ii) the larR(MN)QO operon encodes a transcriptional regulator (LarR) and a four-component ABC-type nickel transporter [Lar(MN), in which the M and N components are fused, LarQ, and LarO]. LarR is a novel regulator of the Crp-Fnr family (PrfA group). Here, the role of LarR was further characterized in vivo and in vitro. We show that LarR is a positive regulator that is absolutely required for the expression of Lar activity. Using gel retardation experiments, we demonstrate that LarR binds to a 16-bp palindromic sequence (Lar box motif) that is present in the larR-larA intergenic region. Mutations in the Lar box strongly affect LarR binding and completely abolish transcription from the larA promoter (PlarA). Two half-Lar boxes located between the Lar box and the -35 box of PlarA promote LarR multimerization on DNA, and point mutations within one or both half-Lar boxes inhibit PlarA induction by l-lactate. Gel retardation and footprinting experiments indicate that l-lactate has a positive effect on the binding and multimerization of LarR, while d-lactate antagonizes the positive effect of l-lactate. A possible mechanism of LarR regulation by lactate enantiomers is proposed

The histidine kinase PdhS controls cell cycle progression of the pathogenic alphaproteobacterium Brucella abortus

Bacterial differentiation is often associated with the asymmetric localization of regulatory proteins, such as histidine kinases. PdhS is an essential and polarly localized histidine kinase in the pathogenic alphaproteobacterium Brucella abortus. After cell division, PdhS is asymmetrically segregated between the two sibling cells, highlighting a differentiation event. However, the function(s) of PdhS in the B. abortus cell cycle remains unknown. We used an original approach, the pentapeptide scanning mutagenesis method, to generate a thermosensitive allele of pdhS. We report that a B. abortus strain carrying this pdhS allele displays growth arrest and an altered DivK-yellow fluorescent protein (YFP) polar localization at the restrictive temperature. Moreover, the production of a nonphosphorylatable PdhS protein or truncated PdhS proteins leads to dominant-negative effects by generating morphological defects consistent with the inhibition of cell division. In addition, we have used a domain mapping approach combined with yeast two-hybrid and fluorescence microscopy methods to better characterize the unusual PdhS sensory domain. We have identified a fragment of the PdhS sensory domain required for protein-protein interaction (amino acids [aa] 210 to 434), a fragment sufficient for polar localization (aa 1 to 434), and a fragment (aa 527 to 661) whose production in B. abortus correlates with the generation of cell shape alterations. The data support a model in which PdhS acts as an essential regulator of cell cycle progression in B. abortus and contribute to a better understanding of the differentiation program inherited by the two sibling cells

Distinct and Specific Role of NlpC/P60 Endopeptidases LytA and LytB in Cell Elongation and Division of Lactobacillus plantarum

Peptidoglycan (PG) is an essential lattice of the bacterial cell wall that needs to be continuously remodeled to allow growth. This task is ensured by the concerted action of PG synthases that insert new material in the pre-existing structure and PG hydrolases (PGHs) that cleave the PG meshwork at critical sites for its processing. Contrasting with Bacillus subtilis that contains more than 35 PGHs, Lactobacillus plantarum is a non-sporulating rod-shaped bacterium that is predicted to possess a minimal set of 12 PGHs. Their role in morphogenesis and cell cycle remains mostly unexplored, except for the involvement of the glucosaminidase Acm2 in cell separation and the NlpC/P60 D, L-endopeptidase LytA in cell shape maintenance. Besides LytA, L. plantarum encodes three additional NlpC/P60 endopeptidases (i.e., LytB, LytC and LytD). The in silico analysis of these four endopeptidases suggests that they could have redundant functions based on their modular organization, forming two pairs of paralogous enzymes. In this work, we investigate the role of each Lyt endopeptidase in cell morphogenesis in order to evaluate their distinct or redundant functions, and eventually their synthetic lethality. We show that the paralogous LytC and LytD enzymes are not required for cell shape maintenance, which may indicate an accessory role such as in PG recycling. In contrast, LytA and LytB appear to be key players of the cell cycle. We show here that LytA is required for cell elongation while LytB is involved in the spatio-temporal regulation of cell division. In addition, both PGHs are involved in the proper positioning of the division site. The absence of LytA activity is responsible for the asymmetrical positioning of septa in round cells while the lack of LytB results in a lateral misplacement of division planes in rod-shaped cells. Finally, we show that the co-inactivation of LytA and LytB is synthetically affecting cell growth, which confirms the key roles played by both enzymes in PG remodeling during the cell cycle of L. plantarum. Based on the large distribution of NlpC/P60 endopeptidases in low-GC Gram-positive bacteria, these enzymes are attractive targets for the discovery of novel antimicrobial compounds